∗rabbit anti-human/mouse/rat p63 polyclonal antibody  (Proteintech)

Journal: Scientific Reports

Article Title: Bio-printing method as a novel approach to obtain a fibrin scaffold settled by limbal epithelial cells for corneal regeneration

doi: 10.1038/s41598-024-73383-y

Figure Lengend Snippet: LESC cells isolated from porcine eye explants and its characterization. Explants of corneoscleral junction ( A , B , C ), LESC cells migrated from explants after 3 days ( D ), confluent culture after 7 days ( E , F , scalebar = 100 µm). Cells with characteristic epithelial cytoskeletal structure visualized by phalloidin staining ( G ). CK14 ( H ) and p63 ( I ) antigens are present in high percentage. Additional composite image with Hoechst staining and CK14 and p63 ( J ) showing that significant fraction of cells are CK14 and p63 positive (scalebar = 200 µm).

Article Snippet: Next, the samples were incubated with primary antibodies including PE-conjugated rabbit monoclonal anti-human p63-α (#56,687, Cell Signaling Technology, diluted 1:100) and FITC-conjugated mouse monoclonal anti-human CK14 (LS-C535410, LsBio, diluted 1:100) at 4 °C overnight.

Techniques: Isolation, Staining

Journal: Scientific Reports

Article Title: Bio-printing method as a novel approach to obtain a fibrin scaffold settled by limbal epithelial cells for corneal regeneration

doi: 10.1038/s41598-024-73383-y

Figure Lengend Snippet: CK14 and p63 positive cells on a fibrin surface after 7 day culture for bioprinted samples as well as for manual method. High percentage of CK14 + and p63 + was noted in all groups. Images from immunostaining show mostly areas partially covered by cell to illustrate morphological differences of expanding epithelia. Differentiated (CK14- and p63-) cells are more abundant on the edges of samples produced by drop method. Scalebar = 100 µm.

Article Snippet: Next, the samples were incubated with primary antibodies including PE-conjugated rabbit monoclonal anti-human p63-α (#56,687, Cell Signaling Technology, diluted 1:100) and FITC-conjugated mouse monoclonal anti-human CK14 (LS-C535410, LsBio, diluted 1:100) at 4 °C overnight.

Techniques: Immunostaining, Produced

Journal: Scientific reports

Article Title: Bio-printing method as a novel approach to obtain a fibrin scaffold settled by limbal epithelial cells for corneal regeneration.

doi: 10.1038/s41598-024-73383-y

Figure Lengend Snippet: Fig. 1. LESC cells isolated from porcine eye explants and its characterization. Explants of corneoscleral junction (A, B, C), LESC cells migrated from explants after 3 days (D), confluent culture after 7 days (E, F, scalebar = 100 µm). Cells with characteristic epithelial cytoskeletal structure visualized by phalloidin staining (G). CK14 (H) and p63 (I) antigens are present in high percentage. Additional composite image with Hoechst staining and CK14 and p63 (J) showing that significant fraction of cells are CK14 and p63 positive (scalebar = 200 µm).

Article Snippet: Next, the samples were incubated with primary antibodies including PE-conjugated rabbit monoclonal anti-human p63-α (#56,687, Cell Signaling Technology, diluted 1:100) and FITC-conjugated mouse monoclonal anti-human CK14 (LS-C535410, LsBio, diluted 1:100) at 4 °C overnight.

Techniques: Isolation, Staining

Journal: Scientific reports

Article Title: Bio-printing method as a novel approach to obtain a fibrin scaffold settled by limbal epithelial cells for corneal regeneration.

doi: 10.1038/s41598-024-73383-y

Figure Lengend Snippet: Fig. 6. CK14 and p63 positive cells on a fibrin surface after 7 day culture for bioprinted samples as well as for manual method. High percentage of CK14 + and p63 + was noted in all groups. Images from immunostaining show mostly areas partially covered by cell to illustrate morphological differences of expanding epithelia. Differentiated (CK14- and p63-) cells are more abundant on the edges of samples produced by drop method. Scalebar = 100 µm.

Article Snippet: Next, the samples were incubated with primary antibodies including PE-conjugated rabbit monoclonal anti-human p63-α (#56,687, Cell Signaling Technology, diluted 1:100) and FITC-conjugated mouse monoclonal anti-human CK14 (LS-C535410, LsBio, diluted 1:100) at 4 °C overnight.

Techniques: Immunostaining, Produced

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: P63 and Ki-67 expression in radicular cyst

doi: 10.1016/j.jobcr.2023.06.008

Figure Lengend Snippet: Expression of P63 and Ki-67, and degree of inflammatory infiltrate in the studied radicular cysts.

Article Snippet: Both primary antibodies were diluted at 1:100 and consisted of polyclonal rabbit anti-human Ki-67 antigen (Catalog No. PB9026, Wuhan Boster Biological Technology, Ltd., Wuhan, China) and polyclonal rabbit anti-human P63 antigen which mainly recognizes ΔNp63 isoforms (Catalog No. BA1326, Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Expressing

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: P63 and Ki-67 expression in radicular cyst

doi: 10.1016/j.jobcr.2023.06.008

Figure Lengend Snippet: Immunohistochemical staining of P63 and Ki-67 in radicular cysts (magnification, ×400). (A) Strong P63 positivity through the epithelium and connective tissue, (B) moderate P63 positivity mainly through the epithelium, (C) strong P63 positivity in the inflammatory cells throughout the connective tissue, (D) absence of P63 staining in the epithelium and connective tissue wall, (E) moderate Ki-67 staining in the nucleus of the positive cells throughout the whole epithelium, and (F) strong Ki-67 positivity throughout epithelium and in many cells in the connective tissue capsule.

Article Snippet: Both primary antibodies were diluted at 1:100 and consisted of polyclonal rabbit anti-human Ki-67 antigen (Catalog No. PB9026, Wuhan Boster Biological Technology, Ltd., Wuhan, China) and polyclonal rabbit anti-human P63 antigen which mainly recognizes ΔNp63 isoforms (Catalog No. BA1326, Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Immunohistochemical staining, Staining

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: P63 and Ki-67 expression in radicular cyst

doi: 10.1016/j.jobcr.2023.06.008

Figure Lengend Snippet: Correlations between P63, Ki-67, inflammatory infiltrate, and size of the studied radicular cysts.

Article Snippet: Both primary antibodies were diluted at 1:100 and consisted of polyclonal rabbit anti-human Ki-67 antigen (Catalog No. PB9026, Wuhan Boster Biological Technology, Ltd., Wuhan, China) and polyclonal rabbit anti-human P63 antigen which mainly recognizes ΔNp63 isoforms (Catalog No. BA1326, Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Expressing

Journal: The Prostate

Article Title: Tissue clearing techniques for three-dimensional optical imaging of intact human prostate and correlations with multi-parametric MRI

doi: 10.1002/pros.24129

Figure Lengend Snippet: Patient 2 is a 53 year-old male with Gleason 4+3 prostate cancer. (a) Whole mount 100 μm thick section before (left) and after (right) clearing using iDISCO. (b) Normal prostate glands from the same prostate area are shown on the thick iDISCO-processed section (left) and on the adjacent H/E slide (right). Similarities in tissue architecture are evident between the two techniques. The red signal from the volume images is the antibody anti-p63. (c) mpMRI images showing ADC (left) and Ktrans (right) maps. The white arrows point in the ADC and Ktrans correspond to the histological regions in (b), reflecting concordance between histology and mpMR for normal prostate. Scale bars are 100 microns.

Article Snippet: The samples were incubated with primary antibodies (mouse anti-human alpha-methylacyl coenzyme-A racemase (AMACR), Thermo Fisher Scientific, Waltham, MA, and rabbit anti-human p63-α (p63), Cell Signaling, Danvers, MA) and with secondary antibody incubation (Cell Signaling, Danvers, MA).

Techniques: